A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of saxagliptin hydrochloride and glibenclamide in synthetic mixture using simultaneous equation method. The absorbance was measured at 210.00 nm for saxagliptin hydrochloride and 229.00 nm for glibenclamide and calibration curves were plotted as absorbance versus concentration, respectively. The method was found to be linear (r2>0.9995) in the range of 5-25 μg/ml for saxagliptin hydrochloride at 210.00 nm. The linear correlation was obtained (r2>0.9996) in the range of 5-25 μg/ml for glibenclamide at 229.00 nm. The limit of determination (LOD) was 0.307 μg/ml and 0.149 μg/ml for saxagliptin hydrochloride and glibenclamide respectively. The limit of quantification (LOQ) was 0.931 μg/ml and 0.452 μg/ml for saxagliptin hydrochloride and glibenclamide respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99%.The method was successfully applied for simultaneous determination of saxagliptin hydrochloride and glibenclamide in binary mixture.
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